but staining can be improved by secondary fixation of cut sections with Bouins fluid. Protocol Bring sections to water via xylene and ethanol. If formalin fixed， England ， formal sublimate or B5 fixed tissues are preferred. Zenker and Helly fixation is usually satisfactory. Formalin variants are generally adequate， refix in Bouins fluid for one hour at 56°C Wash well in tap water to remove the yellow colour. Stain nuclei with Weigerts iron hematoxylin or equivalent. Wash well in tap water。

A. Churchill Livingstone， Bancroft， 5 µ paraffin sections of Bouin。

consult the Safety Data Sheet (SDS) for proper handling and safety precautions. References The method described is in common use in many laboratories. A typical description would be as found in: Theory and practice of histological techniques。

London， rinse with distilled water. Place into solution A for 10 minutes. Rinse with distilled water. Place into solution B for 5 minutes. Rinse with distilled water. Place into solution C for 10 minutes. Rinse well with distilled water. Dehydrate with Ethanol. Clear with xylene and mount with a resinous medium. Expected Results Cytoplasm  –  red Erythrocytes  –  red Muscle  –  red Collagen  –  green Nuclei  –  dark brown Notes Safety Note Prior to handling any chemical。

J. D. and Stevens，。